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Product Description
BHMT Antibody | 46-634 | ProSci
Host: Goat
Reactivity: Human, Mouse, Rat
Homology: Expected Species Reactivity based on sequence homology: Dog
Immunogen: The immunogen for this antibody is: C-EQQLKELFEKQK
Research Area: Signal Transduction
Tested Application: E, WB, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 45kDa band observed in Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45.0kDa according to mouse NP_057877.1 and rat NP_110477.1) . This product has been successfully used in WB on Human (PMID: 27320863) . This product has been successfully used in WB on Mouse (PMID:28605831, 23807810 and 22209966) . This product has been successfully used in WB on Rat (Choi et al. Journal of Functional Foods 44 (2018) 65-73) . In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Kidney. Recommended concentration: 5ug/ml.
Specificiy: N/A
Positive Control 1: Cat. No. 1464 - Rat Liver Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: BHMT, betaine-homocysteine methyltransferase
User Note: N/A
BACKGROUND: N/A
