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Product Description
HNRNPA2B1 Antibody | 43-499 | ProSci
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen: The immunogen for this antibody is: C-DTIEIITDRQS
Research Area: Cancer, Cancer
Tested Application: E, WB, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in lysates of cell line MCF7 and approx. 35kDa in lysates of cell line NIH3T3 and nuclear NIH3T3 (calculated MW of 36.0kDa according to NP_002128.1) . An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.01-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Small Intestine shows nuclear staining. Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Specificiy: This antibody is expected to recognize both reported isoforms (NP_002128.1; NP_112533.1) .
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Approx 36kDa
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, RNPA2, SNRPB1, heterogeneous nuclear ribonucleoproteins A2/B1, hnRNP A2 / hnRNP B1, nuclear ribonucleoprotein particle A2 protein
User Note: N/A
BACKGROUND: N/A
