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Product Description
HSPA8 Antibody | 45-072 | ProSci
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen: The immunogen for this antibody is: C-EKLQGKINDEDKQK
Research Area: Homeostasis
Tested Application: E, IF, WB
Application: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 75kDa band observed in lysates of cell lines HepG2 and NIH3T3 (calculated MW of 70.9kDa according to Human NP_006588.1 and Mouse NP_112442.2) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml
Specificiy: This antibody is expected to recognise isoform 1 (NP_006588.1) only.
Positive Control 1: Cat. No. 1202 - A431 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: HSPA8, heat shock 70kDa protein 8, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP, MGC131511, MGC29929, NIP71, LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70, heat shock 70kD protein 8, heat shock 70kd protein 10, heat shock cognate protein 54, heat shock cognate protein, 71-kDa, lipopolysaccharide-associated protein, uncharacterized bone marrow protein BM034
User Note: N/A
BACKGROUND: N/A
