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Product Description
PTB Antibody | 46-243 | ProSci
Host: Goat
Reactivity: Human, Mouse, Rat
Homology: Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen: The immunogen for this antibody is: DGIVPDIAVGTKR-C
Research Area: Cancer
Tested Application: E, WB, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Human, Mouse and Rat Ovary lysates and 60kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat, HEK293 and 3T3N (calculated MW of 57.2kDa according to NP_114368.1) . Recommended concentration: 0.01-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Liver and Ovary for this particular batch.Immunohistochemistry:In paraffin embedded Human Kidney shows nuclear membrane staining in all the convoluted tubules. Recommended concentration: 2-4ug/ml.
Specificiy: This antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1) .
Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: PTBP1, PTB, polypyrimidine tract binding protein 1, PTB2, PTB3, PTB4, pPTB, HNRPI, PTB-1, HNRNPI, RNA binding protein, heterogeneous nuclear ribonucleoprotein polypeptide I, polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) , HNRNP-I, MGC10830, MGC8461, RNA-binding protein, heterogeneous nuclear ribonucleoprotein I
User Note: N/A
BACKGROUND: N/A
