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Product Description
RBP1 Antibody | 45-136 | ProSci
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Cow
Immunogen: The immunogen for this antibody is: VEGVVCKQVFKKVQ
Research Area: Homeostasis
Tested Application: E, WB, IF, Flow
Application: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 16kDa band observed in NIH3T3 and approx 17kDa in U251 cell lysates. The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. (calculated MW of 22.3kDa according to Human NP_002890.2 and 15.8kDa according to Mouse NP_035384.1) . Recommended concentration: 0.5-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Specificiy: N/A
Positive Control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: RBP1, CRBP, RBPC, CRBP1, CRABP-I, retinol binding protein 1, cellular, retinol-binding protein 1, cellular, CRBPI
User Note: N/A
BACKGROUND: N/A
