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Product Description
RRAD Antibody | 43-008 | ProSci
Host: Goat
Reactivity: Mouse
Homology: Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Pig, Cow
Immunogen: The immunogen for this antibody is: C-HRRSMPVDERDLQ
Research Area: Other
Tested Application: E, WB
Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in Mouse Heart lysates and no 37kDa in the KO lysates (calculated MW of 33.2kDa according to mouse NP_062636.2 and human NP_004156.1) . There is some non-specific background, we call for caution when used for other assays than Western blot. In transfected HEK293 transiently expressing Mouse Rrad (HA tagged) , a band of approx. 45kDa is observed. No bands are observed in transfected HEK293 transiently expressing related Mouse GTPases. Recommended concentration: 0.5-2ug/ml.
Specificiy: Reported variants represent identical protein: NP_004156.1, NP_001122322.1
Positive Control 1: Cat. No. 1210 - 293 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Approx 37 kDa
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: RRAD, Ras-related associated with diabetes, RAD, RAD1, REM3, GTP-binding protein RAD, OTTHUMP00000174805, RAS (RAD and GEM) like GTP binding 3, ras associated with diabetes
User Note: N/A
BACKGROUND: N/A
