Product Description
SBP2 Antibody | 46-339 | ProSci
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen: The immunogen for this antibody is: C-KERQERKQRLQEN
Research Area: Cancer
Tested Application: E, WB, IP, IHC, ICC, IF
Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 120kDa band detected in HFF cell lysates. Data kindly provided by Laura Papp and Kum Kum Khanna. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Papp et al, Mol Cell Biol. 2006 Jul;26 (13) :4895-910; PMID: 16782878) . Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Testis shows granular cytoplasm staining in Leydig cells. Recommended concentration, 3ug/ml.Immunocytochemistry/Immunofluorescence: This antibody has been successfully used in ICC/IF for C33A cells as described in the following paper: Papp et al, Nucleic Acids Res. 2008 Dec;36 (22) :7192-206, PMID: 19004874.
Immunoprecipitation: This antibody has also been successfully used for IP.
Specificiy: N/A
Positive Control 1: HFF Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: SBP2, RP11-89K14.1, SECISBP2, SECIS binding protein 2, DKFZp686C09169, OTTHUMP00000064929, OTTHUMP00000064930, OTTHUMP00000064931, OTTHUMP00000064932, selenocysteine insertion sequence binding protein 2
User Note: N/A
BACKGROUND: N/A