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Product Description
MARCH10 Antibody | 46-938 | ProSci
Host: Goat
Reactivity: Human, Rat
Homology: Expected Species Reactivity based on sequence homology: Cow
Immunogen: The immunogen for this antibody is: C-SRNYPQPRTEE
Research Area: Cancer
Tested Application: E, WB, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 90 kDa band observed in Rat Testis lysates (calculated MW of 90.5 kDa according to Human NP_689811.2 and of 87.9 kDa according to Mouse NP_766156.2) . Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.
Specificiy: This antibody is expected to recognise both reported isoforms (NP_001094345.1; NP_689811.2) , which are identical.
Positive Control 1: Cat. No. 1476 - Rat Testis Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: MARCH10, membrane-associated ring finger (C3HC4) 10, FLJ35757, MARCH-X, RNF190, ring finger protein 190
User Note: N/A
BACKGROUND: N/A
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