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Product Description
NQO1 Antibody | 45-981 | ProSci
Host: Goat
Reactivity: Human, Rat, Pig
Homology: Expected Species Reactivity based on sequence homology: Dog
Immunogen: The immunogen for this antibody is: C-SIPTDNQIKARK
Research Area: Cancer
Tested Application: E, WB, IF, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 28kDa band observed in lysates of Glioblastoma cell line U251 and approx 30kDa band observed in Human Kidney and Lung lysates and in Rat and Pig Kidney lysates (calculated MW of 27.3kDa according to Human NP_001020604.1 and 30.9kDa according to Human NP_000894.1, Rat NP_058696.2 and Pig NP_001153085.1) . Recommended concentration: 0.03-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 and U251 cells. Recommended concentration: 5-10ug/ml.
Specificiy: This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1) .
Positive Control 1: Cat. No. 1302 - Human Lung Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: NQO1, NAD (P) H dehydrogenase, quinone 1, DTD, QR1, DHQU, DIA4, NMOR1, NMORI, diaphorase-4, diaphorase (NADH/NADPH) , diaphorase (NADH/NADPH) (cytochrome b-5 reductase) , NAD (P) H:menadione oxidoreductase 1, dioxin-inducible 1, DT-diaphorase, NAD (P) H menadione oxidoreductase 1, dioxin-inducible, NAD (P) H:Quinone acceptor oxidoreductase type 1, NAD (P) H menadione oxidoreductase 1, dioxin-inducible, NAD (P) H:quinone oxireductase, azoreductase, diaphorase (NADH/NADPH) (cytochrome b-5 reductase) , dioxin-inducible 1, menadione reductase, phylloquinone reductase, quinone reductase 1
User Note: N/A
BACKGROUND: N/A
