Product Description
ANK1 Antibody | 46-725 | ProSci
Host: Goat
Reactivity: N/A
Homology: Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Cow
Immunogen: The immunogen for this antibody is: C-QIVKRASLKRGKQ
Research Area: Homeostasis
Tested Application: E
Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave approx. 19-23 kDa bands in Human Skeletal Muscle lysates after 0.01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the observed 28+30 kDa bands in the literature for this tissue (PMID: 9430667) and given the calculated size of 17.6 kDa according to NP_065211.2. The 19-22 kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificiy: This antibody is expected to recognise isoform 3 (NP_000028.3) and isoform 5 (NP_065211.2) .
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: ANK1, ankyrin 1, erythrocytic, ANK, SPH1, SPH2, ankyrin 1, ankyrin-1, erythrocytic, ankyrin-R
User Note: N/A
BACKGROUND: N/A