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Product Description
ATG5 Antibody | 46-954 | ProSci
Host: Goat
Reactivity: N/A
Homology: Expected Species Reactivity based on sequence homology: Human, Mouse, Cow, Horse
Immunogen: The immunogen for this antibody is: C-SQVINEMQKKDHKQ
Research Area: Autophagy
Tested Application: E
Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx. 38 kDa band in NIH3T3 lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.4 kDa according to NP_004840.1. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: ATG5, ATG5 autophagy related 5 homolog (S. cerevisiae) , APG5, APG5L, ASP, hAPG5, APG5 (autophagy 5, S. cerevisiae) -like, APG5 autophagy 5-like, ATG5 autophagy related 5-like, OTTHUMP00000040507, apoptosis specific protein
User Note: N/A
BACKGROUND: N/A
