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Product Description
BLNK Antibody | 45-025 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen: The immunogen for this antibody is: C-KDSTRLKYAVKVS
Research Area: Immunology
Tested Application: E, WB, IF, Flow
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70-75kDa band observed in lysates of cell line Daudi (calculated MW of 50.5kDa according to NP_037446.1) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the membranes of HepG2 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of Daudi cells. Recommended concentration: 10ug/ml.
Specificiy: This antibody is expected to recognize all reported isoforms.
Positive Control 1: Cat. No. 1224 - Daudi Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: BLNK, SLP-65, B-cell linker, BASH, BLNK-s, Ly57, MGC111051, SLP65, B cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, OTTHUMP00000020167, Src homology 2 domain-containing leukocyte protein of 65 kDa, BLNK-S, LY57, OTTHUMP00000020168
User Note: N/A
BACKGROUND: N/A
