Product Description
ERK2 Antibody | 45-551 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen: The immunogen for this antibody is: CAAGPEMVRGQVF
Research Area: Cancer
Tested Application: E, WB, IF
Application: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 38kDa band observed in HepG2 lysates (calculated MW of 41.4kDa according to NP_002736 and NP_620407) . Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10ug/ml.
Specificiy: This antibody is expected to recognise an epitope corresponding to aa 9-19 of both reported protein isoforms of human ERK2 / MAPK1 protein.
Positive Control 1: Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: MAPK1, ERK2, ERK, p38, p40, p41, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, mitogen-activated protein kinase 1, protein tyrosine kinase ERK2, mitogen-activated protein kinase 2, extracellular signal-regulated kinase 2, extracellular signal-regulated kinase-2
User Note: N/A
BACKGROUND: N/A