Product Description
GCH1 Antibody | 46-847 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen: The immunogen for this antibody is: C-GKVHIGYLPNKQ
Research Area: Signal Transduction, Neuroscience
Tested Application: E, WB, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 37kDa band observed in Human Tonsil lysates. Customer’s experiments gave identical results in lysates of cell line HUVEC with antibodies raised against different parts of the same protein. (calculated MW of 27.9kDa according to NP_000152.1) . Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum) and Small Intestine. Recommended concentration: 5ug/ml.
Specificiy: This antibody is expected to recognize all four isoforms (NP_000152.1 ; NP_001019195.1 ; NP_001019241.1 ; NP_001019242.1)
Positive Control 1: Cat. No. 1314 - Human Thymus Tissue Lysate
Positive Control 2: Cat. No. 1315 - Human Tonsil Tissue Lysate
Positive Control 3: Cat. No. 1369 - Human Lymph node Tissue Lysate
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: GCH1, GTP cyclohydrolase 1 (dopa-responsive dystonia) , DYT5, GCH, GTP-CH-1, GTPCH1, GTP cyclohydrolase 1, guanosine 5'-triphosphate cyclohydrolase I
User Note: N/A
BACKGROUND: N/A