Product Description
HOXD10 Antibody | 46-955 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Horse, Cow
Immunogen: The immunogen for this antibody is: PNRSCRIEQPVTQQ
Research Area: Homeostasis
Tested Application: E, WB, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 37 kDa band observed in Human Kidney lysates (calculated MW of 38.4 kDa according to NP_002139.2) . Recommended concentration: 0.2-0.6ug/ml. Additional bands of unknown identity were also consistently observed at 90+26 kDa. These bands were successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Kidney shows nuclear staining in some epithelial cells of PCT. Recommended concentration, 3-5ug/ml.
Specificiy: N/A
Positive Control 1: Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: HOXD10, homeobox D10, HOX4, HOX4D, HOX4E, Hox-4.4, homeo box 4D, homeo box D10
User Note: N/A
BACKGROUND: N/A