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Product Description
POU5F1B Antibody | 42-581 | ProSci
Host: Goat
Reactivity: N/A
Homology: Expected Species Reactivity based on sequence homology: Human
Immunogen: The immunogen for this antibody is: C-TPGYGSPHFTALYS
Research Area: Transcription
Tested Application: E
Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.6kDa according to NP_001153014.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificiy: This antibody is expected to crossreact to the almost identical OCT4 / POU5F1 (GeneID 5460) .
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: POU5F1B, POU class 5 homeobox 1B, OCT4-pg1, OTF3C, OTF3P1, POU5F1P1, POU5FLC20, POU5FLC8, POU 5 domain protein, POU class 5 homeobox 1 pseudogene 1, POU domain transcription factor Oct-4, POU domain, class 5, transcription factor 1 pseudogene 1, octamer binding protein 3_like sequence
User Note: N/A
BACKGROUND: N/A
