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Product Description
Pro-EGF Antibody | 43-550 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Dog
Immunogen: The immunogen for this antibody is: SRQERVCNIEKNVS
Research Area: Neuroscience, Obesity, Signal Transduction
Tested Application: E, WB, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 160kDa double band observed in Human Prostate lysates (calculated MW of 134kDa according to NP_001954.2) . The two bands represent glycosylated forms of the mature protein. Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin. Recommended concentration: 5ug/ml.
Specificiy: This antibody is expected to recognize all reported isoforms (NP_001954.2; NP_001171601.1; NP_001171602.1) , but not the mature form. The immunizing peptide represents part of the extracellular domain.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Approx 160kDa
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: EGF, epidermal growth factor, HOMG4, URG, OTTHUMP00000162922, OTTHUMP00000219721, OTTHUMP00000219722, beta-urogastrone, pro-epidermal growth factor
User Note: N/A
BACKGROUND: N/A
