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Product Description
SIPA1 Antibody | 46-371 | ProSci
Host: Goat
Reactivity: Human
Homology: N/A
Immunogen: The immunogen for this antibody is: C-ASKQLGSPTADLA
Research Area: Cancer
Tested Application: E, WB
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 150kDa band observed in Human lysates of EBV-immortilized lymphoblastoid (calculated MW of 112kDa according to NP_694985.28 and NP_006738.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem. 1997 Oct 31;272 (44) :28081-8; PMID: 9346962) . Recommended concentration: 1-3ug/ml. An additional band of 55-60kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this product is used for other assays than Western blot.
Specificiy: Both variants represent identical protein (NP_694985.28 and NP_006738.3) .
Positive Control 1: EBV Immortalized Lymphoblastoid Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: SIPA1, SPA1, MGC17037, signal-induced proliferation-associated gene 1, p130 SPA-1, GTPase-activating protein Spa-1, signal-induced proliferation-associated 1, MGC102688, signal-induced proliferation-associated gene 1, signal-induced proliferation-associated protein 1
User Note: N/A
BACKGROUND: N/A
