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Product Description
UPF3A Antibody | 42-211 | ProSci
Host: Goat
Reactivity: N/A
Homology: Expected Species Reactivity based on sequence homology: Human, Cow
Immunogen: The immunogen for this antibody is: C-KETDKQKKIAEKE
Research Area: Signal Transduction
Tested Application: E
Application: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 48kDa band in lysates of cell lines HeLa and Kelly after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 51kDa according to NP_542418.1 and the reported sizes of 65 to 70kDa from different sources. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificiy: This antibody is expected to recognize both isoforms (NP_075387.1; NP_542418.1) .
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: UPF3A, UPF3 regulator of nonsense transcripts homolog A (yeast) , HUPF3A, RENT3A, UPF3, OTTHUMP00000018789, UPF3 regulator of nonsense transcripts homolog A
User Note: N/A
BACKGROUND: N/A
