Product Description
HELT Antibody | 30-613 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HELT.
Research Area: Transcription
Tested Application: E, WB
Application: HELT antibody can be used for detection of HELT by ELISA at 1:312500. HELT antibody can be used for detection of HELT by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Specificiy: N/A
Positive Control 1: Cat. No. 1219 - MCF7 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 36 kDa
Validation: N/A
Isoform: N/A
Purification: Antibody is purified by peptide affinity chromatography method.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration: batch dependent
Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store HELT antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Alternate Name: HELT, HCM1228, HESL, Mgn, bHLHb44
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: HELT belongs to the HEY family. It contains 1 basic helix-loop-helix (bHLH) domain and 1orange domain. HELT is a transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGCG-3'.