Product Description
HFE Antibody | 29-976 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HFE.
Research Area: Membrane, Cancer
Tested Application: E, WB, IHC
Application: HFE antibody can be used for detection of HFE by ELISA at 1:12500. HFE antibody can be used for detection of HFE by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Specificiy: N/A
Positive Control 1: Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 28 kDa, 40 kDa, 28 kDa, 29 kDa, 39 kDa, 30 kDa, 38 kDa, 19 kDa
Validation: N/A
Isoform: N/A
Purification: Antibody is purified by peptide affinity chromatography method.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration: batch dependent
Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store HFE antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Alternate Name: HFE, HFE1, HH, HLA-H, MGC103790, dJ221C16.10.1, MVCD7, TFQTL2
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: HFE is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M) . It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in its gene.The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M) . It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least eleven alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined.