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Product Description
Acetyl-Histone H3-K14 pAb | 22-813 | ProSci
Host: Rabbit
Reactivity: Mouse, Rat
Homology: N/A
Immunogen: A synthetic acetylated peptide corresponding to residues surrounding K14 of human H3
Research Area: Signal Transduction
Tested Application: WB, IHC, IF, IP, ChIP, ChIPseq
Application: WB: 1:1000 - 1:3000
IHC: 1:200 - 1:500
IF: 1:500 - 1:1000
IP: 1:200 - 1:500
CHIP: 1:50 - 1:100
CHIP seq: 1:50 - 1:100
Specificiy: N/A
Positive Control 1: BT-474
Positive Control 2: 293T
Positive Control 3: H3 protein
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Observed: 15kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: H3F3A, H3t, H3.4, H3/g, H3FT
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
