Product Description
ACLY Antibody | 15-947 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 752-1101 of human ACLY (NP_001087.2) .
Research Area: Cancer, Signal Transduction
Tested Application: WB
Application: WB: 1:200 - 1:2000
Specificiy: N/A
Positive Control 1: HepG2
Positive Control 2: HeLa
Positive Control 3: Raji
Positive Control 4: NIH/3T3
Positive Control 5: Mouse brain
Positive Control 6: Mouse heart
Molecular Weight: Observed: 120kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: ACL, ATPCL, CLATP, ATP-citrate synthase, ATP-citrate (pro-S-) -lyase, citrate cleavage enzyme
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440, 000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.