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Product Description
PSMB8 Antibody | 22-875 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 163-272 of human PSMB8 (NP_004150.1) .
Research Area: Cell Cycle
Tested Application: WB, IHC, IF
Application: WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Specificiy: N/A
Positive Control 1: Jurkat
Positive Control 2: HepG2
Positive Control 3: HL-60
Positive Control 4: NCI-H460
Positive Control 5: Mouse liver
Positive Control 6: Mouse spleen
Molecular Weight: Observed: 23-28kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: PSMB8, proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) , D6S216, D6S216E, LMP7, MGC1491, PSMB5i, RING10, beta5i, OTTHUMP00000029420, OTTHUMP00000029421, OTTHUMP00000062981, low molecular weight protein 7, macropain subunit C13, multicatalytic endopeptidase complex subunit C13, protease component C13, macropain) subunit beta type 8 (large multifunctional protease 7) , proteasome beta 8 subunit, proteasome catalytic subunit 3i, proteasome subunit Y2, proteasome subunit beta 5i, proteasome-related gene 7, JMP, ALDD, NKJO
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex) . Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
