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  • SARS-CoV-2 (COVID-19) Spike S1 Antibody [SP422]
  • <strong> Figure 1 Detection of SARS-CoV-2 Variant Proteins with Spike S1 Antibodies by Direct ELISA</strong> Coating Antigen: SARS-CoV-2 full length spike proteins, including WT, UK variant (B.1.1.7) , SA variant (B.1.135) and Brazil (P.1) . Dilution: 1-3000 ng/mL. Incubate at 4 &#730;C overnight. Detection Antibodies: SARS-CoV-2 Spike S1 Antibody, 10-350, 0.1 &#956;g/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr. <strong>Spike S1 antibody (10-350) has low binding affinity for SA variant (B.1.135) and Brazil (P.1) , both of which carry more mutations in RBD as compared to UK variant (B.1.1.7) . </strong>
  • <strong>Figure 2 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Spike in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Spike antibody (10-350, 3 ug/mL) . Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6) . Samples were incubated with primary antibody overnight at 4&#730;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Spike protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.

    • SARS-CoV-2 (COVID-19) Spike S1 Antibody [SP422] | 10-350

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    SKU:
    223-10-350
    Size:
    0.1 mg
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    Product Description

    SARS-CoV-2 (COVID-19) Spike S1 Antibody [SP422] | 10-350 | ProSci

    Host: Rabbit

    Reactivity: Virus

    Homology: N/A

    Immunogen: Recombinant SARS-CoV-2 spike protein, S1 subunit.

    Research Area: Infectious Disease, COVID-19

    Tested Application: E, IHC

    Application: N/A

    Specificiy: N/A

    Positive Control 1: N/A

    Positive Control 2: N/A

    Positive Control 3: N/A

    Positive Control 4: N/A

    Positive Control 5: N/A

    Positive Control 6: N/A

    Molecular Weight: N/A

    Validation: N/A

    Isoform: N/A

    Purification: The antibody was expressed in HEK293F cells, and subsequently obtained from cell supernatant by protein A affinity chromatography.

    Clonality: Monoclonal

    Clone: SP422

    Isotype: IgG, kappa

    Conjugate: Unconjugated

    Physical State: Liquid

    Buffer: PBS pH7.2

    Concentration: batch dependent

    Storage Condition: SARS-CoV-2 (COVID-19) Spike S1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

    Alternate Name: SARS-CoV-2 (COVID-19) Spike S1 Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) , Surface Glycoprotein, Spike protein

    User Note: Optimal dilutions for each application to be determined by the researcher.

    BACKGROUND: N/A

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