Product Description
SARS-COV-2 Nucleocapsid Protein Antibody (biotin) [1B1A9] | 24-043 | ProSci
Host: Rabbit
Reactivity: Virus
Homology: N/A
Immunogen: Hek293 derived SARS-COV-2 Nucleocapsid Ser2-Ala419 Accession #YP_009724397.2
Research Area: Infectious Disease, COVID-19
Tested Application: E
Application: SARS-COV-2 Nucleocapsid Sandwich Elisa:
ELISA Capture: Recommended Concentration:1-4ug/mL, Sample: SARS-CoV-2 Nucleocapsid Protein Antibody(CAP)
ELISA Detection: Recommended Concentration:0.05-0.2ug/mL, Sample: Biotinylated SARS-CoV-2 Nucleocapsid Protein Antibody
Standard:Recommended Concentration: 1.56-100pg/mL, Sample: Recombinant SARS-COV-2 Nucleocapsid Protein with His tag
Specificiy: SARS-COV-2
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Affinity purification
< 1.0 EU/ug of the protein by LAL method.
Clonality: Recombinant Monoclonal
Clone: 1B1A9
Isotype: Rabbit IgG
Conjugate: biotin
Physical State: Liquid
Buffer: Supplied as a 0.2um filtered solution in PBS, pH 7.4 containing SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody at a concentration of 1mg/mL.
Concentration: 1 mg/ml
Storage Condition: This antibody can be stored at 2˚C - 8˚C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20˚C to -80˚C. Preservative-Free.Avoid repeated freeze-thaw cycles.
Alternate Name: SARS-CoV-2 Nucleocapsid Protein, SARS-CoV-2 NP, nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2], novel coronavirus N Protein, novel coronavirus Nucleocapsid Protein, 2019-nCoV Nucleoprotein, 2019-nCoV N, 2019nCoV N, 2019-nCoV N Protein, 2019 peak N Protein, 2019-nCoV nucleocapsid protein.
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.