Euro
USD
British Pound
NULL
Product Description
ASK1 Antibody | 1151 | ProSci
Host: Rabbit
Reactivity: Human
Homology: Predicted species reactivity based on immunogen sequence: Mouse: (100%)
Immunogen: ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1.
The sequence is different from that of mouse by last two amino acids.
The immunogen is located within the last 50 amino acids of ASK1.
Research Area: Apoptosis, Signal Transduction
Tested Application: E, WB, ICC, IF
Application: ASK1 antibody can be used for detection of ASK1 by Western blot at 0.5 μg/mL. A 155 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1214 - SW1353 Cell Lysate
Positive Control 2: Cat. No. 17-002 - A-431 Cell Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 155 kDa
Validation: N/A
Isoform: N/A
Purification: ASK1 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: ASK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Condition: ASK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: ASK1 Antibody: ASK1, MEKK5, MAPKKK5, ASK1, Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, ASK-1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: ASK1 Antibody: Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-α-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
