Euro
USD
British Pound
NULL
Product Description
DFF45 Antibody | 1148 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: DFF45 antibody was raised against a 20 amino acid peptide near the amino terminus of human DFF45.
The immunogen is located within the first 50 amino acids of DFF45.
Research Area: Apoptosis
Tested Application: E, WB
Application: DFF45 antibody can be used for detection of DFF45, DFF35, and one the cleaved fragment by Western blot at 1:1000 to 1:2000 dilution. 45 and 35 kDa bands can be detected in non-apoptotic cells.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 45 and 35 kDa
Validation: N/A
Isoform: N/A
Purification: DFF45 Antibody is Antibody is DEAE purified.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: DFF45 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: DFF45 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: DFF45 Antibody: DFF1, ICAD, DFF-45, DFF1, DFF45, H13, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: DFF45 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently that was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. DFF45/ICAD have short forms that were termed DFF35 and ICADs, respectively. Upon cleavage of DFF45/ICAD, the caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.
