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Product Description
PHAP III Antibody | 3147 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: PHAP III antibody was raised with a synthetic peptide corresponding to amino acids close to carboxy terminus of human PHAP III.
The immunogen is located within amino acids 200 - 250 of PHAP III.
Research Area: Apoptosis
Tested Application: E, WB, IHC-P, IF
Application: PHAP III antibody can be used for detection of PHAP III by Western blot at 1 μg/mL. A band at approximately 35 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human and rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: PHAP III has no cross-reaction to PHAP I and PHAP I2a.
Positive Control 1: Cat. No. 1203 - A549 Cell Lysate
Positive Control 2: Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 3: Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 4: Cat. No. 11-801 - Human Small Intestine Tissue Slide
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 35 kDa
Validation: N/A
Isoform: N/A
Purification: PHAP III Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PHAP III Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: PHAP III antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: PHAP III Antibody: LANPL, LANP-L, Acidic leucine-rich nuclear phosphoprotein 32 family member E, LANP-like protein
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: PHAP III Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7) , which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
