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Product Description
PUMA Antibody | 3043 | ProSci
Host: Rabbit
Reactivity: Human, Rat
Homology: Predicted species reactivity based on immunogen sequence: Mouse: (78.6%)
Immunogen: Anti-PUMA antibody (3043) was raised against a peptide corresponding to 14 amino acids near the amino terminus of human PUMA isoform 1.
The immunogen is located within the first 50 amino acids of PUMA.
Research Area: Apoptosis, Autophagy, Cancer
Tested Application: E, WB, IHC-P, IF
Application: WB: 2-3 μg/mL; IF: 10-20 μg/mL; IHC: 2.5-10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1210 - 293 Cell Lysate
Positive Control 2: Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 3: Cat. No. 10-001 - Human Breast Tissue Slide
Positive Control 4: Cat. No. 1204 – K562 Cell Lysate
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 21kD
Observed: 23kD
Validation:
Independent Antibody Validation (Figure 2) shows similar PUMA expression profile in both human and mouse cell lines detected by two independent anti-PUMA antibodies that recognize different epitopes, 3041 against C-terminus and 3043 against the N-terminus. PUMA proteins are detected in most of the cell lines with different expression levels by the two independent antibodies.
KO Validation (Figure 7, 8) shows lack of PUMA expression in the thymocytes or neurons of PUMA knockout mice.
Animal Species Reactivity (Figure 10) shows PUMA expression in the rat retina detected with anti-PUMA antibodies (3043) .
Isoform: Human PUMA has 4 isoforms, including isoform 1 (193aa, 21kD) , isoform 2 (131aa, 14kD) , isoform 3 (101aa, 10kD) and isofrom 4 (261aa, 27kD) . This antibody detects human isoform 1, and is predicted to detect mouse and rat PUMA (193aa, 21kD for both of them) .
Purification: PUMA Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PUMA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: PUMA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: PUMA Antibody: JFY1, PUMA, JFY-1, Bcl-2-binding component 3
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53 (1) . The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (2) . PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
