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Product Description
RAIDD Antibody | 1117 | ProSci
Host: Rabbit
Reactivity: Human
Homology: Predicted species reactivity based on immunogen sequence: Mouse: (79%)
Immunogen: RAIDD antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human RAIDD.
The immunogen is located within the last 50 amino acids of RAIDD.
Research Area: Apoptosis, Cancer
Tested Application: E, WB, IHC-P, ICC, IF
Application: RAIDD antibody can be used for detection of RAIDD by Western blot at 2 μg/mL. A 22 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1219 – MCF7 Cell Lysate
Positive Control 2: Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 3: Cat. No. 10-401 - Human Kidney Tissue Slide
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 22 kDa
Observed: 18 kDa
Validation: N/A
Isoform: N/A
Purification: RAIDD Antibody is Antibody is DEAE purified.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: RAIDD Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Condition: RAIDD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: RAIDD Antibody: MRT34, RAIDD, Death domain-containing protein CRADD, Caspase and RIP adapter with death domain
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: RAIDD Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain (DD)-containing receptors, TNFR1 and Fas. The death signals are transduced by a group of DD-containing adapter molecules. A novel cell death adapter was recently identified by two independent groups and designated RAIDD (RIP-associated ICH-1/CED-3-homologous protein with DD) and CRADD (caspase and RIP adapter with DD)1, RAIDD contains a DD and a CARD (for caspase recruitment domain) which interact with RIP and caspase, respectively, to transduce death signals. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR-1.
