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![Recombinant PMEL17 / Melanoma gp100 Antibody [PMEL/1825R]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/575614/812594/porsci_lo__79508.1648973713__78246.1649091982.png?c=2 "Recombinant PMEL17 / Melanoma gp100 Antibody [PMEL/1825R]")
![Recombinant PMEL17 / Melanoma gp100 Antibody [PMEL/1825R]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/575614/812594/porsci_lo__79508.1648973713__78246.1649091982.png?c=2 "Recombinant PMEL17 / Melanoma gp100 Antibody [PMEL/1825R]")
Product Description
Recombinant PMEL17 / Melanoma gp100 Antibody [PMEL/1825R] | 33-816 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Recombinant human protein was used as the immunogen for the recombinant gp100 antibody.
Research Area: Cancer, Obesity, Signal Transduction
Tested Application: Flow, IF, IHC-P
Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 0.5-1 ug/ml for 30 min at RT
Prediluted format: incubate for 30 min at RT (1)
Optimal dilution of the recombinant gp100 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein A affinity chromatography
Clonality: Recombinant Monoclonal
Clone: PMEL/1825R
Isotype: IgG, k
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Melanocyte protein PMEL, ME20-M, ME20M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, M-alpha, 95 kDa melanocyte-specific secreted glycoprotein, P26, Secreted melanoma-associated ME20 antigen, ME20-S, ME20S, M-beta, PMEL, D12S53E, PMEL17, SILV
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: Cytotoxic T lymphocytes (CTL's) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein.
![Recombinant PMEL17 / Melanoma gp100 Antibody [PMEL/1825R]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575614/812594/porsci_lo__79508.1648973713__78246.1649091982.png?c=2 "Recombinant PMEL17 / Melanoma gp100 Antibody [PMEL/1825R]")
