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Product Description
ORC1 Antibody | 15-710 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 696-856 of human ORC1 (NP_001177748.1) .
Research Area: Cell Cycle
Tested Application: WB
Application: WB: 1:500 - 1:2000
Specificiy: N/A
Positive Control 1: HeLa
Positive Control 2: 293T
Positive Control 3: A-431
Positive Control 4: K-562
Positive Control 5: Jurkat
Positive Control 6: MCF7
Molecular Weight: Observed: 90kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: HSORC1L, PARC1, ORC1, origin recognition complex subunit 1, origin recognition complex, subunit 1 homolog, replication control protein 1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1) , a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
