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Product Description
B-raf Antibody | 5777 | ProSci
Host: Chicken
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Chicken: (93%)
Immunogen: Anti-B-raf antibody (5777) was raised against a peptide corresponding to 18 amino acids near the center of human B-raf.
The immunogen is located within amino acids 330 - 380 of B-raf.
Research Area: Cancer, Cell Cycle
Tested Application: E, WB
Application: WB: 0.5-2 μg/mL.
Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2: Cat. No. 1210 – 293 Cell Lysate
Positive Control 3: Cat. No. 1224 – Daudi Cell Lysate
Positive Control 4: Cat. No. 1204 – K562 Cell Lysate
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 94kD
Observed: 94 kD
Validation:
Independent Antibody Validation in Cell lines (Figure 2) shows similar B-raf expression profile in human and mouse cell lines detected by two independent anti-B-raf antibodies that recognize different epitopes, 5777 against central domain and 7379 against a slight different central domain. B-raf proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.
Isoform: Human B-raf has one isoform (766aa, 84kD) . Mouse B-raf has 2 isoforms, including isoform 1 (804aa, 89kD) and isoform 2 (329aa, 37kD) . Rat B-raf has one isoform (712aa, 80kD) . 5777 can detect in human B-raf and mouse isoform 1.
Purification: B-raf Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgY
Conjugate: Unconjugated
Physical State: Liquid
Buffer: B-raf Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: B-raf antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: B-raf Antibody: NS7, BRAF1, RAFB1, B-RAF1, Proto-oncogene B-Raf
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS) , a disease characterized by heart defects, mental retardation and a distinctive facial appearance.
