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Product Description
CANX Antibody | 42-774 | ProSci
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen: The immunogen for this antibody is: C-SKTPELNLDQFHDKT
Research Area: Other
Tested Application: E, WB, IHC, IF, EIA
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 100kDa band observed in Mouse Heart, Human Brain (Cerebellum) , NIH3T3 and HeLa lysates (calculated MW of 67.6kDa according to Human NP_001737.1 and 67.2kDa according to Mouse NP_001103969.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Oliver et al., J Biol Chem. 1996 Jun 7;271 (23) :13691-6., PMID: 8662691) . Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows textured staining in the cytoplasm of DCT cells. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Testis and Uterus. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 5ug/ml.
Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Calnexin captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Specificiy: Reported variants represent identical protein (NP_001019820.1, NP_001737.1) .
Positive Control 1: Cat. No. 1401 - Mouse Heart Tissue Lysate
Positive Control 2: Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 3: Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 4: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: CANX, calnexin, CNX, FLJ26570, IP90, P90, major histocompatibility complex class I antigen-binding protein p88
User Note: N/A
BACKGROUND: N/A
