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Product Description
G6PD Antibody | 45-644 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen: The immunogen for this antibody is: C-STNSDDVRDEKVK
Research Area: Cancer, Infectious Disease
Tested Application: E, WB, IHC, EIA
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55-60kDa double band observed in Human Testis, Thyroid and Tonsil lysates (calculated MW of 62.5kDa according to NP_000393.4 and 59.3kDa accordiong to NP_001035810.1) . Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis and Spleen. Recommended concentration: 2.5ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant G6PD captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Specificiy: This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1) .
Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: G6PD, glucose-6-phosphate dehydrogenase, G6PD1, glucose-6-phosphate 1-dehydrogenase, glucose-6-phosphate dehydrogenase, G6PD
User Note: N/A
BACKGROUND: N/A
