Product Description
ERCC1 Antibody | 42-738 | ProSci
Host: Goat
Reactivity: Human
Homology: N/A
Immunogen: The immunogen for this antibody is: DPGKDKEGVPQPS-C
Research Area: Other
Tested Application: E, WB
Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 38kDa band observed in lysates of cell lines A431 and Kelly (calculated MW of 35.6kDa according to NP_973730.1) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Specificiy: This antibody is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1) .
Positive Control 1: Cat. No. 1202 - A431 Cell Lysate
Positive Control 2: KELLY (Human neuroblastoma cell line) Lysate
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: ERCC1, excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence) , COFS4, UV20, excision repair cross-complementing 1, excision repair protein
User Note: N/A
BACKGROUND: N/A