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Product Description
IL-20 Antibody | XP-5184 | ProSci
Host: Goat
Reactivity: Human
Homology: N/A
Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL-20 (human Interleukin-20) .
Research Area: Immunology, Chemokines & Cytokines, Antibody Pairs
Tested Application: E, WB
Application: ELISA:
To detect hIL-20 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-20.
Sandwich:
To detect hIL-20 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-20 (38-133) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-20.
Western Blot:
To detect hIL-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Anti-hIL-20 specific antibody was purified by affinity chromatography employing immobilized hIL-20 matrix.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Lyophilized
Buffer: N/A
Concentration: N/A
Storage Condition: IL-20 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Alternate Name: IL-20, IL10D, ZCYTO10, UNQ852/PRO1801, Interleukin-20, Cytokine Zcyto10, IL-20
User Note: Centrifuge vial prior to opening.
BACKGROUND: IL20 is a cytokine structurally related to interleukin 10 (IL10) . This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.
