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Product Description
IL-6 Antibody (biotin) | 38-202 | ProSci
Host: Goat
Reactivity: Human
Homology: N/A
Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL-6. Human IL-6 specific antibody was purified by affinity chromatography and then biotinylated.
Research Area: Immunology, Chemokines & Cytokines, Neuroscience, Antibody Pairs
Tested Application: E, WB
Application: ELISA:
Sandwich:
To detect hIL-6 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IL-6 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6.
Western Blot:
To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Biotin
Physical State: Lyophilized
Buffer: N/A
Concentration: N/A
Storage Condition: IL-6 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Alternate Name: HGF, HSF, BSF2, IL-6, IFNB2, Interleukin-6, B-cell stimulatory factor 2
User Note: Centrifuge vial prior to opening.
BACKGROUND: Interleukin 6 (IL-6) is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL-6 has also been implicated in regulating adipose mass.
