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Product Description
IRAK4 Antibody | 45-074 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse
Immunogen: The immunogen for this antibody is: NKPITPSTYVRC
Research Area: Immunology
Tested Application: E, WB, IHC, ICC, Flow
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in lysates of cell line HeLa and approx 55kDa in lysates of cell line Jurkat (calculated MW of 51.5kDa according to NP_057207.1) . This molecular weight is routinely observed by other sources. Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Heart and Human Liver. Recommended concentration: 3.75µg/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm and microtubules of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Specificiy: N/A
Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: IRAK4, REN64, NY-REN-64, interleukin-1 receptor-associated kinase 4, IRAK-4 mutated form 1, interleukin-1 receptor associated kinase 4, interleukin-1 receptor associated kinase 4 mutant form 1, IPD1
User Note: N/A
BACKGROUND: N/A
