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Product Description
NOX5 Antibody | 42-714 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen: The immunogen for this antibody is: C-EWHPFTISSAPEQKD
Research Area: Other
Tested Application: E, IF, WB
Application: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 90kDa band observed in Human Testis lysates (calculated MW of 86.4kDa according to NP_078781.3) . Recommended concentration: 0.5-2ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the plasma membrane and cytoplasm of HeLa and MCF7 cells. Recommended concentration:10ug/ml.
Specificiy: This antibody is expected to recognize all reported isoforms (NP_078781.3; NP_001171708.1; NP_001171709.1) .
Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: NOX5, NADPH oxidase, EF-hand calcium binding domain 5, MGC149776, MGC149777, NADPH oxidase 5, OTTHUMP00000164443, OTTHUMP00000164444
User Note: N/A
BACKGROUND: N/A
