Product Description
NQO1 Antibody | 42-451 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen: The immunogen for this antibody is: C-DKGPFRSKKAVLS
Research Area: Cancer
Tested Application: E, WB, IF, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 30kDa band observed in Human Kidney lysates (calculated MW of 30.9kDa according to NP_000894.1) . Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 5ug/ml.
Specificiy: This antibody is expected to recognize reported isoform a (NP_000894.1) only.
Positive Control 1: Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: NQO1, NAD (P) H dehydrogenase, quinone 1, DHQU, DIA4, DTD, NMOR1, NMORI, QR1, DT-diaphorase, NAD (P) H dehydrogenase [quinone] 1, NAD (P) H:Quinone acceptor oxidoreductase type 1, NAD (P) H:menadione oxidoreductase 1, NAD (P) H:quinone oxidoreductase 1, NAD (P) H:quinone oxireductase, azoreductase, diaphorase (NADH/NADPH) (cytochrome b-5 reductase) , diaphorase-4, dioxin-inducible 1, menadione reductase, phylloquinone reductase, quinone reductase 1
User Note: N/A
BACKGROUND: N/A