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Product Description
PRDM1 Antibody | 42-784 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow, Pig
Immunogen: The immunogen for this antibody is: C-DISDNADRLEDVED
Research Area: Other
Tested Application: E, WB, IF, Flow
Application: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 90kDa band observed in lysates of cell lines A431 and Nuclear K562, and approx. 100kDa in lysates of cell line K562. An additional faint band of approx 70kDa was also seen in some A431 and K562 lysates (calculated MW of 91.8kDa according to NP_001189.2 isoform 1 and 76.8kD according to NP_878911.1 isoform 2) . Recommended concentration: 0.1-2g/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Specificiy: This antibody is expected to recognize both reported isoforms (NP_001189.2; NP_878911.1) .
Positive Control 1: Cat. No. 1202 - A431 Cell Lysate
Positive Control 2: Cat. No. 1203 - A549 Cell Lysate
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: PRDM1, PR domain containing 1, with ZNF domain, BLIMP1, MGC118922, MGC118923, MGC118924, MGC118925, PRDI-BF1, B-lymphocyte-induced maturation protein 1, OTTHUMP00000016918, PR-domain zinc finger protein 1, PRDI-binding factor-1, beta-interferon gene positive-regulatory domain I binding factor, positive regulatory domain I-binding factor 1
User Note: N/A
BACKGROUND: N/A
