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Product Description
SPAG16 Antibody | 42-304 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen: The immunogen for this antibody is: C-KLHYASYEPTIR
Research Area: Other
Tested Application: E, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 55kDa and 22kDa in Human Testis lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 70.8kDa according to NP_078808.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) .Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.
Specificiy: This antibody is expected to recognize isoform 1 (NP_078808.3) only.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: SPAG16, sperm associated antigen 16, DKFZp666P1710, FLJ22724, FLJ37717, MGC87036, PF20, WDR29, WD repeat domain 29, sperm-associated WD repeat protein
User Note: N/A
BACKGROUND: N/A
