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Product Description
Alpha-tubulin Antibody | 7175 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%) , Pig: (100%) , Chicken: (100%)
Immunogen: Rabbit polyclonal Tubulin antibody was raised against a 16 amino acid peptide near the amino terminus of human Tubulin.
The immunogen is located within amino acids 140 - 190 of Alpha-tubulin.
Research Area: Homeostasis
Tested Application: E, WB
Application: Tubulin antibody can be used for detection of Tubulin by Western blot at 0.5 and 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 50 kDa
Validation: N/A
Isoform: N/A
Purification: Alpha-tubulin Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Alpha-tubulin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: Alpha-tubulin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: Alpha-tubulin Antibody: TUBA2, bA408E5.3, TUBA2, TUBA3D, Alpha-tubulin 2
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Alpha-tubulin Antibody: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots.
