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Product Description
LAMTOR1 Antibody | 7709 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen: Rabbit polyclonal LAMTOR1 antibody was raised against a 17 amino acid peptide near the amino terminus of human LAMTOR1 .
The immunogen is located within the first 50 amino acids of LAMTOR1.
Research Area: Homeostasis
Tested Application: E, WB, IHC-P, IF
Application: LAMTOR1 antibody can be used for detection of LAMTOR1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: LAMTOR1 antibody is human, mouse and rat specific. LAMTOR1 antibody is predicted to not cross-react with other LAMTOR family proteins.
Positive Control 1: Cat. No. 1202 - A431 Cell Lysate
Positive Control 2: Cat. No. 11-801 - Human Small Intestine Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 18 kDa
Observed: 18 kDa
Validation: N/A
Isoform: N/A
Purification: LAMTOR1 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: LAMTOR1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: LAMTOR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: LAMTOR1 Antibody: p18, PDRO, C11orf59, p27RF-Rho, Ragulator1, PP7157, Ragulator complex protein LAMTOR1, Lipid raft adaptor protein p18
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: LAMTOR1 is a 161 amino acid membrane protein belonging to the LAMTOR family. It has been identified to interact with and recruit the four Rag GTPases (Rag A-D) to the surface of lysosomes (1) . The mTORC1 kinase complex is a critical component in the regulation of cell growth (2) . As part of the ragulator complex, LAMTOR1 recruits the Rag GTPases and the mTORC1 complex to lysosomes, a key step in activation of the TOR signaling cascade by amino acids (3) . LAMTOR1 may be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes / lysosomes (4) .
