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Product Description
Nephrin Antibody | 2265 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Anti-Nephrin antibody (2265) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Nephrin.
The immunogen is located within the last 50 amino acids of Nephrin.
Research Area: Homeostasis
Tested Application: E, WB, IHC-P, IF
Application: WB: 1-2 μg/mL; IHC: 1-5 μg/mL; IF: 10-20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 2: Cat. No. 1203 - A549 Cell Lysate
Positive Control 3: Cat. No. 1285 - C2C12 Cell Lysate
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation:
Induced Expression Validation (Figure 10) : Nephrin expression detected by anit-Nephrin antibodies was up-regulated by curcumin treatment in type 1 diabetic rats.
Localization Validation (Figure 11) : Nephrin detected by anti-Nephrin antibodies is colocalized with PKGIα at the tips of the cell processes in rat podocytes.
Regulated expression validation (Figure 14) : Nephrin expression detected by anit-Nephrin antibodies was down-regulated by PEGF treatment (NGP or HGP) , which was reversed by the addition of C3 transferase in mouse podocyte cells.
Isoform: Human Nephrin has 2 isoforms, including isoform 1 (1241aa, 135kD) and isoform 2 (1201aa, 131kD) . Mouse Nephrin also has 2 isoforms, including isoform 1 (1256aa, 136kD) and isoform 2 (1242aa, 135kD) . Rat Nephrin has 3 isoforms, including isoform 1 (1252aa, 136kD) , isoform 2 (1239aa, 135kD) and isoform 3 (1166aa, 127kD) . 2265 can detect isoforms of human, mouse and rat.
Purification: Nephrin Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Nephrin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: Nephrin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: Nephrin Antibody: CNF, NPHN, nephrin, Nephrin, Renal glomerulus-specific cell adhesion receptor
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Nephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.
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