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Product Description
PIG-Y Antibody | 4955 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%) , Rat: (94%)
Immunogen: PIG-Y antibody was raised against a 16 amino acid synthetic peptide near the center of human PIG-Y.
The immunogen is located within amino acids 50 - 100 of PIG-Y.
Research Area: Homeostasis
Tested Application: E, WB, IHC-P, IF
Application: PIG-Y antibody can be used for detection of PIG-Y by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: This antibody only detects isoform 1 of PIG-Y.
Positive Control 1: Cat. No. 1304 – Human Liver Tissue Lysate
Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 8 kDa
Observed: 16 kDa
Validation: N/A
Isoform: N/A
Purification: PIG-Y Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PIG-Y Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: PIG-Y antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: PIG-Y Antibody: PIG-Y, Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y, Phosphatidylinositol-glycan biosynthesis class Y protein, PIG-Y
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: PIG-Y Antibody: Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT) , a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.
