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Product Description
RAB21 Antibody | 6927 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (95%)
Immunogen: RAB21 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human RAB21.
The immunogen is located within the last 50 amino acids of RAB21.
Research Area: Homeostasis
Tested Application: E, WB, IF
Application: RAB21 antibody can be used for detection of RAB21 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: This antibody is predicted to not cross-react with other Ras-related proteins
Positive Control 1: Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: RAB21 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: RAB21 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: RAB21 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: RAB21 Antibody: KIAA0118, Ras-related protein Rab-21
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: RAB21 Antibody: RAB21 is a member of a subfamily of small GTP-binding protein genes of the Ras superfamily that plays an important role in intracellular vesicular targeting. In non-polarized Caco-2 cells, RAB21 showed a general endoplasmic reticulum (ER) -like localization, while in polarized cells, RAB21 localized to apical vesicles. RAB21 has been shown to associate with integrin subunits and to be important for receptor entry into cells via RAB5/RAB21 endosomes. The GTPase-activating protein p120RasGAP regulates cell motility by controlling the return of the endocytosed integrins to the plasma membrane by competing with RAB21 for binding to overlapping sites on the α-tail of endocytosed integrin.
