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Product Description
SPRYD4 Antibody | 6407 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (89%) , Pig: (89%)
Immunogen: SPRYD4 antibody was raised against an 18 amino acid synthetic peptide near the center of human SPRYD4.
The immunogen is located within amino acids 70 - 120 of SPRYD4.
Research Area: Homeostasis
Tested Application: E, WB, IHC-P, IF
Application: SPRYD4 antibody can be used for detection of SPRYD4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: SPRYD4 antibody is predicted to not cross-react with other SPRYD protein family members. At least two isoforms of SPRYD4 are known to exist; this antibody will detect both isoforms.
Positive Control 1: Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: SPRYD4 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: SPRYD4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: SPRYD4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: SPRYD4 Antibody: SPRY domain-containing protein 4
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: SPRYD4 Antibody: The SPRY domain-containing protein 4 (SPRYD4) is a member of a family of proteins whose sole common characteristic is the presence of a SPRY domain. SPRY domains are structural domains that were first described in the fungal Dictyostelium discoideum tyrosine kinase spore lysis A. In most systems SPRY domains provide binding sites for regulatory proteins or intramolecular binding sites that maintain the structural integrity of a protein. SPRYD4 is ubiquitously expressed and is most abundant in kidney, brain, bladder, thymus and stomach. Little is known of the function of the SPRYD4 protein.
